Sanger's methodSanger’s method,whichis also referred to as dideoxy sequencing or chain termination, is ... based on the use of dideoxynucleotides (ddNTP’s) in addition to the normal nucleotides (NTP’s) found ... stranded DNA5'3'① DNA labeled at 5'ends with polynucleotide kinase and 32p labeled ATPⓟ --ⓟ② separation
