coli JM109){Streak E.coli on LB plate↓Incubate at 37℃, O/N↓Inoculate a single colony into LB broth ... 이를 E.coli에 넣어 증식시킨 다음 Lac selection (blue/white colony) 법에 의해 recombinant, transformants를 선택한다. ... (4㎖)↓Incubate at 37℃, with shaking at 200rpm, O/N↓Inoculate 40㎕ of the cell culture into a 4㎖ fresh medium