분생보고서-DNA extraction

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분생보고서-DNA extraction
분생실험에서의 DNA 추출에 관해 다룬 보고서 입니다.

목차

1. Introduction
2. Materials and Method
3. Results
4. discussion

본문내용

1. Introduction
What is the DNA extraction?
- DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis.
Outline of a DNA extraction
- There are three basic steps in a DNA extraction, the details of which may vary depending on the type of sample and any substances that may interfere with the extraction and subsequent analysis.
Chelate divalent cations such as Mg2+ and Ca2+ to stop dnase enzymes functioning and degrading the DNA
Break open cells by grinding or sonication , and remove membrane lipids by adding a detergent .
Remove cellular and histone proteins bound to the DNA, by adding a protease , by precipitation with sodium or ammonium acetate, or by using a phenol-chloroform extraction step.
Precipitate DNA in cold ethanol or isopropanol , DNA is insoluble in alcohol and clings together; this step also removes salt.
Wash the resulting DNA pellet with alcohol
Solubilize the DNA in a slightly alkaline buffer
2. Materials and Method
- Materials
1M Tris, 10% SDS, EDTA, D.W, Homogenizer, protease K, 얼음, phenol, NaCl, High speed -centrifuge, kidney 조직
- Method
sol 1: 0.1ml(Tris)+0ml(SDS)+2ml(EDTA)+7.9ml(D.W)
sol 2: 0.1ml(Tris)+1ml(SDS)+2ml(EDTA)+6.9ml(D.W)

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