[생의학]Tail DNA preparation
- 최초 등록일
- 2007.02.06
- 최종 저작일
- 2004.11
- 4페이지/
한컴오피스
- 가격 1,000원
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소개글
생의학시간에 실시한 실험입니다.
목차
■ Principle
■ Apparatus & Reagent
■ Producer & Method
■ Result & Discussion
본문내용
■ Principle
This procedure was developed to analyze large numbers of mouse tail DNA samples. It has been found to work well with restriction enzymes. It has now been adapted to isolate DNA from mouse extra-embryonic membrane. Isolated DNA is currently used as template in PCR
■ Apparatus & Reagent
mouse tail, 8M Potassium acetate, Tail lysis buffer, Proteinase K, Tail resuspension buffer (TE), 100% ethanol, chloroform, 70% EtOH
vortex, centrifuge, pipet
■ Producer & Method
- Label 2 sets of tubes.
- Store the tails at 20℃, if not processed immediately.
1. Prepare a master mix
- Tail lysis buffer with proteinase K: 400 ㎕/tail (1:10 dilution of stock enzyme (10 mg/ml) in lysis buffer to get 1 mg/ml.
2. Set the incubation at 65℃ overnight.
참고 자료
1. http://science.empas.com
2. http://www.protocol-online.org/
3. 김수일 외 5인, 생화학, 문운당, 1990
4. 생물학연구정보센터 ( http://bric.postech.ac.kr )