Signal transduction Pathways Regulated by Mitogen-activated/Extracellular Response Kinase Kinase Kinase Induce Cell Death
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소개글
MEKK selectively regulates signal transduction pathways that contribute to the apoptotic response.
목차
HYPOTHESIS
MEKK selectively regulates signal transduction pathways that contribute to the apoptotic response.
Figure1. Morphological changes induced by expression of
activated MEKK (MEKKCOOH)
Table 1. Quantitation of MEKKCOOH induced cell death
Figure 3. (A) Expression of MEKKCOOH induces cytoplasmic
shrinkage and nuclear condensation
Figure 3. (B) Expression of MEKKCOOH induces cytoplasmic
shrinkage and nuclear condensation
Figure 4. Detection of DNA fragments in Swiss 3T3 cells
expression MEKKCOOH
Figure 5. Wild-type Swiss 3T3 cells were transfected with pCMV5BXBRaf or pCMV5 without a cDNA insert in the presence of expression plasmids encoding Gal4/Elk-1 and Gal4-TK-luciferase as described under “Materials and Methods.”
Figure 6. (A) Induction of MEKKCOOH expression in
Swiss 3T3 cells increases the number of
condensed cells.
Figure 6. (B) Induction of MEKKCOOH expression in
Swiss 3T3 cells increases the number of
condensed cells.
Figure 6. (C) Induction of MEKKCOOH expression in Swiss 3T3 cells increases the number of condensed cells.
Figure 7 . (A) Assay of MEKK-regulated protein kinases in
Swiss 3T3 cells.
Figure 7. (b) Assay of MEKK-regulated protein kinases in
Swiss 3T3 cells.
Figure 7. (C) Assay of MEKK-regulated protein kinases in
Swiss 3T3 cells.
Figure 8. Competitive inhibitory JNK/SAPK(APF) attenuates
Gal4/Jun but not Gal4/Myc activation.
Figure 9. Expression of JNK/SAPK(APF) does not affect the
MEKKCOOH induced cellular condensation response.
SUMMARY
CONCLUSION
본문내용
Expression of activated MEKK induces cell death.
Induction of activated MEKK sensitizes Swiss 3T3 cells to UV-induced apoptosis.
Expression of MEKKCOOH stimulates JNK/SAPK and the transactivation of c-Myc and Elk-1.
Inhibitory JNK/SAPK does not attenuate MEKK-stimulated c-Myc transactivation or cell condensation
참고 자료
THE JOURNAL OF BIOLOGICAL CHEMISTRY
Vol.271, NO. 6. Issue of February 9. pp. 3229~3237 , 1996 Printed in U.S.A.