[유전공학]제조합 단백질 생산을 위한 host cell

소개글

제조합 단백질 생산을 위한 host cell...숙주세포로 사용할 수 있는 것들의 종류와 그 특징, post translational system의 장단점에 대한 내용

목차

General problems with the production of recombinant protein in E. coli
Production of recombinant protein by eukaryotic cells

본문내용

1. Problems resulting from the sequence of the foreign gene
① foreign gene might contain introns
② foreign gene might contain termination signal ⇒ premature termination
③ foreign gene might contain unfavored codons

2. Problems caused by E. coli
① might not process the recombinant protein correctly e.g. glycosylation
② might not fold the recombinant protein correctly e.g. disulfide bond, inclusion body
③ might degrade the recombinant protein

Production of recombinant protein by eukaryotic cells

1. Recombinant protein from yeast and filamentous fungi
Saccharomyces cerevisiae
① high yield
② unable to glycosylate animal proteins correctly, adding to many sugar unit
③ lacks an efficient secreting system
④ accepted as a safe organism for production of protein for use in medicines, foods

참고 자료

gene cloning