면역학 ELISA 실험 레포트

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"면역학 ELISA 실험 레포트"에 대한 내용입니다.

목차

1.실험제목(title)
2.실험날짜(date):
3.실험목적(purpose):
4.실험 준비물(material-reagent, instrument)
5.실험 방법(method)
6.실험 결과(result)
7.실험 고찰(discussion)
8.참고문헌(reference)

본문내용

· 실험 제목(title): ELISA assay(mouse macrophagy from bone marrow)

· 실험날짜(date):

· 실험목적(purpose): 항원-항체 특이적 결합반응과 효소를 이용한 발색반응을 통해 target을 정성, 정량적으로 분석하는 방법을 익히기 위함이다.

· 실험 준비물(material-reagent, instrument)
pipet, assay diluent, tip, L929 sup와 LPS를 모두 처리한 well의 상층액, PBST, working detector(Detection Antibody+SAv-HRP reagent), stop solution(H3PO4),

· 실험 방법(method)
① coating과 blocking과정을 거친 well을 준비한다.

참고 자료

Kenneth Murphy, Paul Travers, Mark Walport, 『Janeway's immunobiology』, 라이프사이언스, 7판, ,78-79p, 727-728p
Toshio Tanaka, Masashi Narazaki, Tadamitsu Kishimoto, IL-6 in Inflammation, Immunity, and Disease, Cold Spring Harb Perspect Biol. 2014 Oct; 6(10)
https://terms.naver.com/entry.nhn?docId=490884&cid=60408&categoryId=55558
https://en.wikipedia.org/wiki/Immunoassay
https://terms.naver.com/entry.nhn?docId=426099&cid=60261&categoryId=60261
https://terms.naver.com/entry.nhn?docId=927655&cid=51007&categoryId=51007
https://terms.naver.com/entry.nhn?docId=491321&cid=60408&categoryId=55558
https://terms.naver.com/entry.nhn?docId=484613&cid=60408&categoryId=55558
https://en.wikipedia.org/wiki/ELISA
https://www.bosterbio.com/newsletter-archive/20170728-which-elisa
http://www.dynebio.co.kr/yc/bbs/board.php?bo_table=data1&wr_id=13&sst=wr_hit&sod=desc&sop=and&page=1
www.woongbee.com/0e-DM/2014/JE/4/ELISAworkshop.pdf
https://en.wikibooks.org/wiki/Structural_Biochemistry/Proteins/Enzyme-Linked_Immunoabsorbent_Assay_(ELISA)#Reverse_ELISA
https://rockland-inc.com/ELISA-Kits.aspx
https://terms.naver.com/entry.nhn?docId=420891&cid=60261&categoryId=60261
http://www.ijbs.com/Interleukin-6.htm