[FPLC] FPLC
- 최초 등록일
- 2004.06.07
- 최종 저작일
- 2004.06
- 27페이지/ MS 파워포인트
- 가격 2,000원
소개글
powerpoint 슬라이드 문서입니다.
FPLC에대한 발표자료입니다.
영어로 작성되어있습니다.
목차
FPLC-Liquid Chromatography System for Fast Protein Purification
HPLC-High Performance Liquid Chromatography
Base Material for HPLC
Gel Filtration Chromatography
IEC, AC & HIC Chromatography
IEC, AC & HIC Chromatography
Chromatography Methods
Four Major Properties
Four Classic Chromatography
Gel Filtration Chromatography
Ion Exchange Chromatography
Characteristics
IEC media
Functional groups
Hydrophobic Interaction Chromatography
Characteristics
HIC media
Hydrophobic Ligands
Affinity Chromatography
Characteristics
AC media
Coupling to NHS-activated Gel
Polyhistidine tagged fusion protein purification using chelating gel
Human IgG purification using Protein A gel
Purification Strategy
Application
본문내용
Ion Exchange Chromatography
Characteristics
- Change of surface charge with solution pH
NH2 -> NH3+, COOH -> COO-
pI : isoelectric point, net charge is zero.
- Cationic proteins are bound to cation exchange gel.
- Elution : gradient or step methods with high salt buffer
IEC media
- Base material : agarose (Sepharose), cellulose (Sephacell), synthetic polymer
- Manufacturer : Pharmacia, Millipore, Waters, Bio-Rad
- Strong & weak ion exchangers
참고 자료
없음