유전학2_경북대

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유전학2
chapter 10 proteomics - chapter13(예정)
12/15(목)
chapter10
[ gene expression 측정 방법 2가지]
1. DNA microarray
what?
a collection of microsopic dna attached to solid surface
2 dna chips and differnce?
A chip arrayed with oligonucleotides synthesized
directly on the chip
A chip arrayed with denatured
meaning of result of hybridization?
which sample is overexpressed or underexpressed relative to
another sample in gene
benefits?
even very rare transcrips can be detected
sensitive to relativelt small diffences in levels of transcript abundance
limitations?
hybridization efficiencies can be different by probes
requires prior sequence knowledge to design chip
2. RNA-seq
benefits?
each transcript is identified according to sequence
no hybridization involved
no prior knowledge of genomes sequence necessary

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