Exp 1. Quenching Study with Absorption and Fluorescence Spectroscopy
- 최초 등록일
- 2022.06.02
- 최종 저작일
- 2022.04
- 10페이지/ MS 워드
- 가격 3,500원
소개글
"Exp 1. Quenching Study with Absorption and Fluorescence Spectroscopy"에 대한 내용입니다.
목차
1. Abstract
2. Introduction
3. Materials
4. Methods
5. Results and Discussion
6. Conclusion
본문내용
Abstract
Luminescence quenching occurs when the excited molecule or atom releases energy in a form of light as the relaxation process takes place. In this experiment, we used UV/vis spectrometer and fluorescence spectrometry to understand the mechanism of fluorescence quenching. Since the quencher reduces the fluorescence, we could observe decreasing fluorescence intensity depending on the concentration of the quencher. The fluorescence yield for coumarin 1 was 4.56373 and the second-order quenching constant was 3.32*108 M-1s-1. Comparing this value with the diffusion rate constant 2.777*108 M-1s-1, there was a 19.55% error
Introduction
Fluorescence is the luminescence caused by the absorption of radiation at a certain wavelength. When the fluorescence intensity is decreased by adding a quencher, it is called fluorescence quenching. This can happen by excited-state reactions, molecular rearrangements, energy transfer, and ground-state complex formation.
참고 자료
https://wiki.anton-paar.com/kr-kr/sulfuric-acid/