[식물] Chloroplast pigmentary separation

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목차

1.Principles
2.Materials
3.Procedure
4.Points
5.Reference

본문내용

(Procedure)

1.spinach leaf 2 - 3 put in water that boil and kill cell.
2.Having picked up leaf, after clean water and put to triturator , put and go perfectly when 2-3ml remains dividing abstraction solvent 15ml 2-3 times.
3.Cut filter paper by 2.0*8.0cm, and draw line of crosswise with pencil in position that become 2㎝ from the end.
4.Dry after imprint several times on width smearing abstraction solution into toothpick.
5.After fill some developing solvent to test tube, block entrance by aluminum foil and do so that test tube interior may be gotten saturated in developing solvent.
6.End of filter paper may be reach about 0.5㎝ in developing solvent. This time, point of abstraction solution should hold up with developing solvent.
7.Take out and confirm points that is detached just before developing solvent arrives on the filter paper top portion.
8.Calculate each Rf values.

참고 자료

www.ncbi.com
kimbyungchul college biology pp.34-38