[영문] 분자생물학

목차

Peptidomics technologies for human body fluids
1. Extending proteomics with technologies for the analysis of peptides
2. Extracellular fluids in humans
3. Peptides in blood plasma
4. Other body fluid
5. Quantitative approaches
6. Differential display of complex mixtures
7. Prospect

본문내용

* Peptides play a central role in many physiological processes.
* The use of technologies other than 2D gel in order to analyse all peptides and small
proteins.
* Using liquid chromatography or mass spectrometry have been developed and applied to
the analysis of human body fluids.

The establishment of human genome as the blueprint for human life
- Necessary : the human proteome project
→ Understanding of the molecules that make up our complex regulatory systems
Search for the biochemical messenger molecules
- Regulatory processes : peptide hormones, neuropeptides, cytokines and enzyme
inhibitors
- Communication between all cell types through body fluids
Significant progression in the field of proteomics
- The sequences of secretin and insulin (the first examples)
- Several important classes of peptide hormones :
gut hormones, neuropeptides, leuteinizing hormone releasing hormone, growth hormone
Peptides are of paramount importance ( Table 1 )
The lack of technologies that enable a comprehensive analysis of peptides
- Purifications from tissue extracts guided by function
- Many steps are needed to generate a new sequence

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