A+보장, 생화학 RNA 추출 및 정량 예비레포트

소개글

약학대학 과정의 생화학 실험 레포트입니다.
(다른 자연과학 생화학 실험의 과정과 거의 유사합니다.)
긴말하지 않겠습니다.
A+ 받았던 예비보고서 입니다.
받아보시면 후회하지 않으실겁니다.
관련 자료들을 모아 정리해 놓은 것이기 때문에 웬만한 내용은 거의 포함하고 있습니다.

목차

1. 실험제목 : RNA Preparation & Quantification

2. 이론 및 관련 원리
1) RNA의 종류, 역할, 특징을 조사하시오.
2) RNA의 추출 원리와 RNA의 순도/농도 측정법에 대해 조사하시오.
3) RNA 취급시의 주의점에 대해 조사하시오.

3. References

본문내용

1. 실험제목 : RNA Preparation & Quantification

2. 이론 및 관련 원리
1) RNA의 종류, 역할, 특징을 조사하시오.
(RNA의 종류 – 개관>)
⓵ Coding RNA – mRNA
: Messenger RNA (mRNA) is the RNA that carries information from DNA to the ribosome, the sites of protein synthesis (translation) in the cell. The coding sequence of the mRNA determines the amino acid sequence in the protein that is produced.

⓶ Non-coding RNA – tRNA, rRNA
- miRNA, RNAi, siRNA, piRNA
- CRISPR RNA, Antisense RNA, Xist
- snRNA, snoRNA
- Viroids / dsRNA
: Many RNAs do not code for protein (about 97% of the transcriptional output is non-protein-coding in eukaryotes). These so-called non-coding RNAs ("ncRNA") can be encoded by their own genes, but can also derive from mRNA introns. The most prominent examples of non-coding RNAs are transfer RNA (tRNA) and ribosomal RNA (rRNA), both of which are involved in the process of translation. There are also non-coding RNAs involved in gene regulation, RNA processing and other roles.

참고 자료

http://en.m.wikipedia.org/wiki/RNA
http://en.m.wikipedia.org/wiki/RNA
http://en.wikipedia.org/wiki/Messenger_RNA#Structure
http://en.m.wikipedia.org/wiki/RNA
http://en.m.wikipedia.org/wiki/Transfer_RNA#Structure
http://www.ncbi.nlm.nih.gov/books/NBK21603/
http://en.m.wikipedia.org/wiki/Ribosomal_RNA#Eukaryotes
http://en.m.wikipedia.org/wiki/MicroRNA
http://en.m.wikipedia.org/wiki/MicroRNA#Nomenclature
http://en.m.wikipedia.org/wiki/Small_interfering_RNA#Structure
http://en.m.wikipedia.org/wiki/Piwi-interacting_RNA#Function
http://en.m.wikipedia.org/wiki/Piwi-interacting_RNA#Characteristics
http://en.m.wikipedia.org/wiki/XIST_(gene)
http://en.m.wikipedia.org/wiki/XIST_(gene)#Transcript_organization
http://www.nature.com/scitable/topicpage/rna-functions-352 (paragraph - Small Nuclear RNAs)
http://en.m.wikipedia.org/wiki/Small_nuclear_RNA
http://en.m.wikipedia.org/wiki/Small_nucleolar_RNA
http://bioscience.egloos.com/m/5279387
http://nogarded.egloos.com/m/4071499
http://openwetware.org/wiki/RNA_extraction_using_trizol/tri
http://www.cbil.upenn.edu/downloads_orig/EPConDB/download/Protocols/RNA%20-%20Evaluating%20Concentration%20and%20Purity.pdf
http://cms.takara.co.kr/file/lsnb/20_h_1.pdf
http://bric.postech.ac.kr/myboard/read.php?Board=exp_qna&id=65861