단백질 정량(3주차) 결과 레포트

목차

1. 실험의 목적
2. 실험 결과
3. Discussion
4. Further study
5. Reference

본문내용

1. Bradford assay에서 발색이 되어 595nm에서 흡광도가 변하는 생화학적 기전은 무엇인가?
Coomassie Brilliant Blue R-250 dying reagent is the most convenient and fast way to dye polyacrylamide gel. And it is ready-to-use so does not need to measure or mix again. In acidic solution, Coomassie Brilliant Blue R-250 combines with protein and because of it, the maximum absorbance of dye shifts from 465 nm to 595 nm. Thus at the 595 nm, absorbance is proportional to the concentration of protein.

참고 자료

https://www.thermofisher.com/kr/ko/home/brands/thermo-scientific/pierce-protein-biology.html#/legacy=piercenet.com
http://agvod.snu.ac.kr/journal/sej/full/p0513_051104.pdf
http://yasuar.hihome.com/wordhtm/proteinassay.htm