분자생물학기초실험/ 분자생명공학기초실험 8주차: Protein Overexpression with Escherichia coli [A+ 리포트]
- 최초 등록일
- 2017.01.25
- 최종 저작일
- 2016.11
- 7페이지/
어도비 PDF
- 가격 3,600원
![할인쿠폰받기](/images/v4/document/ico_det_coupon.gif)
소개글
연세대학교 2016년 2학기 A+ 받은 리포트입니다.
실험관련 topic에 대해 많은 조사를 했기에 이 자료 하나로 충분할거에요.
목차
1. Objective
2. Experimental Background
3. Method
4. Results/Discussion
5. Quiz
6. References
본문내용
<Experimental Background>
At current stage, Escherichia coli (E. coli) is the most well-known expression system in various bioprocesses used for production of the recombinant proteins such as therapeutic proteins and industrial enzymes. This is because E. coli is genetically well characterized with rapid replication or growth using cost-effective medium as well as due to mutant host strains and number of expression vectors are available. So in this particular experiment, gene of interest is cloned into pET21a-EGFP-His expression vector that is then transformed into competent E.coli.
<Conclusion>
In conclusion, the objective of this experiment was to learn recombinant protein production in bacterial expression systems as well as to measure the optical density of cells and fluorescent quantification. Measurements were done to ultimately observe the effect of IPTG concentration on cell growth curve and GFP expression.
참고 자료
An Introduction to Fluorescence Resonance Energy Transfer (FRET) Technology and Its Application in Bioscience - LabAutopedia. Web. 13 Nov. 2016. <http://www.labautopedia.org/mw/An_Introduction_to_Fluorescence_Resonanc https://arxiv.org/e_Energy_Transfer_(FRET)_Technology_and_its_Application_in_Bioscience>.
Basic Molecular Biotechnology Laboratory Booklet – Department of Biotech. Yonsei University.
Madigan, Michael T., John M. Martinko, Jack Parker, and Thomas D. Brock. Brock Biology of Microorganisms. Upper Saddle River, NJ: Prentice Hall, 2012. Print.
Patrick, Marcy. "Plasmids 101: Antibiotic Resistance Genes." Plasmids 101: Antibiotic Resistance Genes. Web. 08 Oct. 2016. <http://blog.addgene.org/plasmids-101-everything-you-need-to-know-aboutantibiotic-resistance-genes>.