[생화학실험] Bradford법 pre-report (예비레포트)
- 최초 등록일
- 2016.06.16
- 최종 저작일
- 2010.03
- 3페이지/ 한컴오피스
- 가격 1,000원
* 본 문서는 한글 2005 이상 버전에서 작성된 문서입니다.
한글 2002 이하 프로그램에서는 열어볼 수 없으니, 한글 뷰어프로그램(한글 2005 이상)을 설치하신 후 확인해주시기 바랍니다.
소개글
생화학실험 Bradford Reagent 실험 예비레포트 입니다.
영문으로 쓰여져 있습니다.
목차
1. Principle
2. Reagents & Equipments
reference
본문내용
1. Principle
The Bradford assay is one of measuring method how many protein is contained in the sample. The Bradford assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts when binding to protein occurs.
During the formation of this complex, two types of bond interaction take place: the red form of coomassie dye first donates its free electron to the ionizable groups on the protein, which causes a disruption of the protein's native state, consequently exposing its hydrophobic pockets.
참고 자료
http://blog.naver.com/ckurapika0?Redirect=Log&logNo=10048124339
http://en.wikipedia.org/wiki/Bradford_protein_assay
http://www.cyworld.com/immunoplay/158082
http://www.ruf.rice.edu/~bioslabs/methods/protein/bradford.html
http://www.piercenet.com/products/browse.cfm?fldID=02020105