RNA electrophoresis using formaldehyde agarose gel
- 최초 등록일
- 2014.09.13
- 최종 저작일
- 2014.08
- 3페이지/
한컴오피스
- 가격 1,000원
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목차
Ⅰ. Introduction
Ⅱ. Materials
Ⅲ. Method
Ⅳ. Reference
본문내용
Ⅰ. Introduction
Agarose gels containing formaldehyde
RNA is fractionated by electrophoresis through an agarose gel containing 2.2 M formaldehyde. Formaldehyde forms unstable Schiff bases with the single imino group of guanin residues. These adducts maintain RNA in the denatured state by preventing intrastrand Watson-Crick base pairing. Solution containing formaldehyde should be handled with great care and only in a chemical fume hood.
≪특징≫ ① slimier
② less elastic
③ more frangible
≪장점≫ The denaturant is more easily dissociated than is glyoxal from RNA after electrophoresis
≪단점≫ The bands of RNA in formaldehyde-agarose gels are fuzzy and do not match the crisp beauty of bands in glyoxal-agarose gels.
The high concentration of denaturant compensates for loss of formaldehyde by diffusion from the gel into the buffer during electrophoresis. ( Solution ) ⇒ Running gels for shorter times at higher voltages (7-10 V/cm), which allows the concentration of formaldehyde in the gels to be reduced to 1.1% or 0.66 M.
참고 자료
Sambrook and Russell, 2001, Molecular cloning 3rd, CSHL press, volume1. : 7.31~7.34