RNA electrophoresis using formaldehyde agarose gel

목차

Ⅰ. Introduction
Ⅱ. Materials
Ⅲ. Method
Ⅳ. Reference

본문내용

Ⅰ. Introduction

Agarose gels containing formaldehyde
RNA is fractionated by electrophoresis through an agarose gel containing 2.2 M formaldehyde. Formaldehyde forms unstable Schiff bases with the single imino group of guanin residues. These adducts maintain RNA in the denatured state by preventing intrastrand Watson-Crick base pairing. Solution containing formaldehyde should be handled with great care and only in a chemical fume hood.
≪특징≫ ① slimier
② less elastic
③ more frangible

≪장점≫ The denaturant is more easily dissociated than is glyoxal from RNA after electrophoresis
≪단점≫ The bands of RNA in formaldehyde-agarose gels are fuzzy and do not match the crisp beauty of bands in glyoxal-agarose gels.

The high concentration of denaturant compensates for loss of formaldehyde by diffusion from the gel into the buffer during electrophoresis. ( Solution ) ⇒ Running gels for shorter times at higher voltages (7-10 V/cm), which allows the concentration of formaldehyde in the gels to be reduced to 1.1% or 0.66 M.

참고 자료

Sambrook and Russell, 2001, Molecular cloning 3rd, CSHL press, volume1. : 7.31~7.34