Western blot
- 최초 등록일
- 2014.03.11
- 최종 저작일
- 2012.03
- 3페이지/
한컴오피스
- 가격 1,000원
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목차
1. Objective
2. Materials
3. Materials
4. Results
5. Discussion
4. Method
5. Discussion
본문내용
1. Objective : western blot을 이용한 항원 항체 이해하고, 각 시약들의 역할을 숙지하며 직접 실험하도록 한다.
2. Materials
장치 : 영동조, 영동판(gel판), comb, 전원장치, electrotransfer Kit, Mitrocellulose Filter
reagent
Acrylamide stock solution (30% acrylamide & 0.8% bis-acrylamide solution), Gel buffer (Separating gel& Stacking gel), 10% sodium dodecylsulfate (SDS), N, N, N', N'-Tetramethylethylenediamine (TEMED), 10% Ammonium Persulfate (APS), Distilled water (D.W.), Protein molecular weight marker, 5X sample buffer, Electrophoresis buffer, Coomassie gel stain solution, Destaining solution, Transfer buffer, PBS buffer, Blocking buffer, primary antibody, secondary antibody
참고 자료
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