Restriction enzyme digestion
- 최초 등록일
- 2013.07.13
- 최종 저작일
- 2011.10
- 6페이지/ 한컴오피스
- 가격 2,000원
목차
1. Title
2. Object
3. Introduction
4. Material & Methods
5. Result
6. Discussion
7. Reference
본문내용
1. Title : Restriction Enzyme digestion
2. Object
Understanding the mechanism of Restriction Enzyme
3. Introduction
[Definition of Restriction Enzyme]
A Restriction Enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA at specific recognition nucleotide sequences known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses. Inside a bacterial host, the restriction enzymes selectively cut up foreign DNA in a process called restriction; host DNA is methylated by a modification enzyme (a methylase) to protect it from the restriction enzyme’s activity. Collectively, these two processes form the restriction modification system. To cut the DNA, a restriction enzyme makes two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
<중 략>
②. about 5 sec, centrifuge the tube briefly to gather materials for reaction at the bottom of tube.
③. at 37℃, to cut out DNA into fragments by Restriction Enz, do reaction for appropraite time (about 1~2 hr and it depends on species of Restriction Enz)
④. Check the reaction using agarose gel electrophoresis.
참고 자료
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RestrictionEnzymes.html
http://www.thelabrat.com/restriction/XhoI.shtml
http://en.wikipedia.org/wiki/HindIII
http://en.wikipedia.org/wiki/Restriction_enzyme
http://www.protocol-online.org/biology-forums/posts/39973.html
http://www.protocol-online.org/cgi-bin/htsearch?config=htdig_pol&restrict=&exclude=&words=restriction+enzyme+Hind+XhoI