site directed mutagenesis
- 최초 등록일
- 2012.10.29
- 최종 저작일
- 2011.09
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소개글
1. site directed mutagenesis란?
(개념 및 구체적인 원리)
2. site directed mutagenesis protocols(구체적인 실험방법들)
3. site directed mutagenesis example.
목차
1. conception
2. principle
3. material and method
4. application of the method in study
본문내용
* conception
“ PROTEIN FUNCTION IS DETERMINED BY STRUCTURE”
1) Domain - structure and function in Protein
2) the major role part & motif
3) DNA sequence insertion, changes, deletion: activate the motif In vitro
2. Using E.coli DAM methylase – DNA methylation
1) Mutant strand synthesis
Perform thermal cycling to
- denathre DNA template
- anneal mutagenic primers containing desired mutation
- extend and incorporate primers with PfuUltra DNA polymerase
2) Dpnl digestion of temolate
Digest parental methylated and hemimethylated DNA wite Dpnl
참고 자료
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