HPLC교육

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HPLC교육 (영문ppt)입니다.

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What is LC?
A chromatographic technique in which the mobile phase is a liquid
LC is a much older technique than GC, but was overshadowed by the rapid development of GC in the 1950’s and 1960’s
LC is currently the dominate type of chromatography and is even replacing GC in its more traditional applications
Purpose of LC
Analytical - determine chemical composition of a sample
Preparative - purify and collect one or more components of a sample
Problem:
What would be a good method for determining the following:
identity of accelerant at a suspected arson scene
amount of caffeine in Coca Cola
identifying active ingredient in an illicit drug preparation (LSD is heat sensitive)
purification and characterization of novel thermophilic plant enzyme from South America
identifying explosive materials used in Oklahoma bombing
Advantages of LC compared to GC
LC can be applied to the separation of any compound that is soluble in a liquid phase.
LC more useful in the separation of biological compounds, synthetic or natural polymers, and inorganic compounds
Liquid mobile phase allows LC to be used at lower temperatures than required by GC
LC better suited than GC for separating compounds that may be thermally labile
Most LC detectors are non-destructive
Low Performance Liquid Chromatography
broad peaks
poor limits of detection
long separation times
columns can only tolerate low operating pressures
Solvent reservoir
Column head
Column
Column packing
Porous glass plate
Column chromatography an example of the equipment used in low-performance liquid chromatography
Sample is usually applied directly to the top of the column.
Detection is by fraction collection

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