[생리학]【A+】pH,buffer

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목차

1. PREPARATION OF PHOSPHATE AND TRIS BUFFERS
2. Buffering action
3. Weak acid and weak Base인 기능기의 양자 평형
4. Henderson-Hasselbalch equation
5. 완충용액(buffer solution)

본문내용

pH and Buffer
1. PREPARATION OF PHOSPHATE AND TRIS BUFFERS
1) PRINCIPLE:
A buffer is a chemical system that tends resist changes in pH when excess acid or base is affected to the system. Buffers are usually made from weak acids which are effective because of the limited dissociation of the weak acid. The Henderson-Hasselbalch equation related the pH of a solution to the pKa of the chemical species and to the ratio of conjugated base to conjugated acid.
2) REAGENTS and APPARATUS
① Potassium phosphate monobasic (KH2PO4)
② Potassium phosphate dibasic (K2HPO4) or Na2HPO4. 12H2O
③ 0.1M HCl and 0.1M NaOH solution (100 ml each)
④ Tris(hydroxymethyl)-aminomethane [Tris]
⑤ pH meter
⑥ Volumetric flasks (100 ml, 50 ml), pipetts (10 ml), beakers (100 ml, 200ml), spoids etc.
⑦ Balance (analytical or table top)
3) Procedures
① Phosphate buffer (0.1M, pH 7.2, 500 ml)
⑴ Prepare 100 ml of 0.1M KH2PO2 (MW: 136) and 0.1M Na2HPO4. 12H2O (MW: 358).
⑵ Mix 250 ml 0.1M KH2PO2 and 250 ml 0.1M N

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