RNA extraction

소개글

RNA extraction 에 관한 이론과 실험에 관한 ppt

목차

Introduction – Trizol and Kit method

Extraction procedure
1. 시료준비 (Cell, Tissue)
2. Extraction (Trizol, Kit)
3. Quantitation and Quality check

Trouble shooting

본문내용

TRIZOL Reagent (U.S.Patent No.5,346,994) is a ready-to-use reagent for the isolation of total RNA from cells and tissues.

The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method developed by Chomczynski and Sacchi1.

During sample homogenization or lysis, TRIZOL Reagent maintains the integrity of the RNA, acting as a strong RNase inhibitor, while disrupting cells and dissolving cell components.

RNeasy Kits represent a novel technology for RNA isolation. This technology combines the selective binding properties of a silica-gel-based membrane with the speed of spin technology. A specialized high-salt buffer system allows up to 100ug(Mini), 1 mg (midi) or 6 mg (maxi) of RNA longer than 200 bases to adsorb to the RNeasy silica-gel membrane.

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