DNA methylation analysis

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DNA methylation analysis 이론과 실험 방법, 정량하는 실험들에 대한 비교 ppt

목차

1. DNA methylation analysis
Southern Blot
Combined Bisulfite Restriction Analysis (COBRA)
Bisulfite treatment
Methylation-specific PCR
Bisulfite sequencing
 
2. Quantitative DNA methylation analysis
Pyrosequencing
Methylight

본문내용

DNA Methylation
The dinucleotide CpG is notably under-represented in the human
genome, only ~20% of the expected frequency. However, there are
many regions (~1kb long) where the frequency of CpG is about 10
times higher than the genome average. Such regions are called
CpG islands.

.Advantages

Speed

Simplicity

No gels or tags are needed 
Certainty of the information

Real sequence information is easy to publish and to communicate to other research labs
Assay design is simple and flexible, with a high chance of first-run success

Disadvantages
Pyrosequencing typically reads at most 40 to 50 nucleotides

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