PCR의 원리와 이용-RNA 정제

소개글

PCR의 원리와 이용-RNA 정제 발표 레포트

목차

Introduction
Yield and Quality of RNA
Exogenous RNase Contamination
Sample processing
RNA Isolation Strategies
Protocol

본문내용

Yield and Quality of RNA
Yield
⇒ Typically assessed by measuring the absorbance of the RNA
solution at 260 nm.
⇒ one A260 unit = RNA 40 ug/ml
Quality
⇒ A260 / A280 ratio (between 1.9 and 2.2)
lower ratio ; contamination with protein,phenol, guanidinium salt.
⇒ Visualization of RNA sample on agarose gel
Formaldehyde gel
⇒ To provide yield and quality
⇒ Dyes : comparison with a standard or computer imaging
⇒ Sensitive ~ 5 nanogram

참고 자료

PCR Primer a Laboratory Manual