Preparation of Tris-HCL Buffer
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- 2010.03.28
- 최종 저작일
- 2009.06
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Preparation of Tris-HCL Buffer
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본문내용
1) Reagent and Materials
0.1 M Tris Base (Trizma;SIGMA Co.)
0.1 N Hydrochloric acid
2L distilled water
pH meter, stirrer, magnetic bar, beakers
Pipette, Kim Wipe, Balancer, spoons, paraffin papers
2) Stock Solutions
A: 0.1M Tris Base solution 500ml (6.055g/500ml)
Pour the 400ml of distilled water into 0.5 litter-beaker
Add the 6.055g of Tris-Base measure by balancer
Melt the base completely using magnetic bar on the stirrer
Fill the distilled water up to 500ml
B: 0.1N Hydrochloric acid solution 500ml (conc. HCL 4.31ML/500ML)
Pour the 495.7ml of distilled water into 0.5 litter-beaker
Add the 4.31 ml of the concentrated hydrochloric acid
3) Adjustment of the appropriate pH
This 50 mM buffers of the desired pH can be made by mixing 50ml of 0.1M Tris base with the indicated volume of 0.1N HCL.
참고 자료
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