translation quality control에 관한 논문 소개 프레젠테이션
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- 2009.06.10
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- 2009.06
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소개글
Quality control by the ribosome following peptide formation이라는 주제의 논문을 해석하고 발표한 자료입니다.
목차
Translation
Fidelity of protein synthesis
Fidelity measurements in their laboratory
Buffers used
In vitro bacterial translation system
Ribosome nascent chain complex (RNC)
Unusual behavior after a miscoding event
Release assays
Enzyme kinetics
Is this an authentic ribosome-based event?
RF3 stimulates promiscuous release by RF2
P site mismatches promote errors in tRNA selection
Amplification of errors leads to fast termination
A mismatch in the E site undergoes a frameshift
A miscoding exited the E site is not identified by RF2
In vivo relevance of post-peptidyl quality control
Conclusions
본문내용
Elongation Factor Thermo unstable
Elongation Factor GTPase
동영상 http://pubs.acs.org/cen/coverstory/85/8508cover.html
Aminoacylation of tRNAs with their cognate amino acid
Specific anticodon in tRNA
그 외의 recognition regions: CCA terminal 근처에 있는 acceptor stem 등에서의 base 차이
혹은 tRNA synthetase의 3D structure
http://www.cs.stedwards.edu/chem/Chemistry/CHEM43/CHEM43/tRNA/Function.htm
http://www.cs.stedwards.edu/chem/Chemistry/CHEM43/CHEM43/tRNA/reg&con.htm#REF
CCA 3`-terminal, acceptor stem, D arm, T arm, anticodon arm
Rate of misincorporation
적으면 amino acid 1600에서 1700개 중에 하나, 많으면 200개 중 하나 정도 되는 확률
참고 자료
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