Preparation and application of graphene oxide nanocomposites loaded with enzymes and liposomes
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서지정보
ㆍ발행기관 : 한국탄소학회
ㆍ수록지정보 : Carbon letters / 33권 / 4호
ㆍ저자명 : Yifan Yu, Ruyuan Wang, Meilin Li, Shanshan Li, Aiping Fan
ㆍ저자명 : Yifan Yu, Ruyuan Wang, Meilin Li, Shanshan Li, Aiping Fan
목차
Preparation and application of graphene oxide nanocomposites loaded with enzymes and liposomesAbstract
Graphical abstract
1 Introduction
2 Materials and methods
2.1 Materials
2.2 Equipment
2.3 Preparation of liposomes
2.4 Preparation of PBA-GOx and PBA-HRP
2.5 Preparation of GO nanocomposites
2.6 Colorimetric determination of glucose
3 Results and discussions
3.1 Preparation of GO-GHL nanocomposites
3.2 Verify the mobilization of liposomes and enzymes on GO
3.3 Feasibility of the one-step colorimetric detection of glucose
3.4 Optimization of preparation conditions for the GO-GHL nanocomposites
3.5 Optimization of experimental conditions for glucose detection
3.6 Detection of glucose
3.7 Detection of glucose in real samples
3.8 Stability of the GO-GHL nanocomposite
4 Conclusion
Anchor 22
Acknowledgements
References
영어 초록
The present studies explored the possibility of immobilizing phosphocholine (PC) liposomes on the surface of graphene oxide (GO) which was pre-adsorbed with two kinds of enzymes, horseradish peroxidase and glucose oxidase. The transmission electron microscope images showed that the PC liposomes adsorbed onto the GO surface kept integrity. By using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-encapsulated liposomes, a one-step colorimetric assay for glucose was developed. In the presence of glucose, the GO nanocomposites catalyzed the cascade enzymatic reaction producing colorimetric signals directly. Under the optimal conditions, the GO nanocomposites produced linearly increased colorimetric signal with increased concentrations of glucose ranging from 50 to 500 μM. The detection limit was 33 μM. The GO nanocomposites also exhibited good selectivity for the detection of glucose and were able to detect glucose in human serum.참고 자료
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