Melia azedarach extract exhibits a broad spectrum of antiviral effect in vitro and in vivo
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서지정보
ㆍ발행기관 : 충북대학교 동물의학연구소
ㆍ수록지정보 : Journal of Biomedical and Translational Research / 21권 / 3호
ㆍ저자명 : N. A. Nadeeka Nethmini, Myun Soo Kim, Kiramage Chathuranga, Jin Yeul Ma, Hongik Kim, Jong-Soo Lee
ㆍ저자명 : N. A. Nadeeka Nethmini, Myun Soo Kim, Kiramage Chathuranga, Jin Yeul Ma, Hongik Kim, Jong-Soo Lee
목차
IntroductionMaterials and Methods
Preparation of Melia azedarach
Cells and viruses
Inhibition of virus replication in Melia azedarachtreated epithelial and macrophage cells
Virus titration of treated cell and cell supernatant
Detection of IFN-β and IL-6 in Melia azedarachtreated cells by enzyme-linked immunosorbentassay (ELISA)
Quantitative RT-PCR (qRT-PCR)
Effects of Melia azedarach on Type I IFN relatedprotein phosphorylation in RAW264.7 cells (Immunoblotanalysis)
Oral administration of Melia azedarach and viralchallenge in BALB/c mice
Lung virus titer
Statistical analysis
Results
Pre-treatment effect of Melia azedarach in differentcell lines
Co-treatment effect of Melia azedarach in differentcell lines
Post-treatment effect of Melia azedarach in differentcell lines
Melia azedarach induces the secretion of IFNs, apro-inflammatory cytokine, and activates the signalingmolecules of the type I IFN and NF-κBsignaling pathway
Melia azedarach induces IFN and pro-inflammatorycytokines gene transcription in the treated cells
Orally-administrated Melia azedarach enhances thesurvival of influenza A virus-infected mice
Discussion
References