Protective effect of stevia plant extract against experimental colon carcinogenesis in mice
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서지정보
ㆍ발행기관 : 충북대학교 동물의학연구소
ㆍ수록지정보 : Journal of Biomedical and Translational Research / 21권 / 4호
ㆍ저자명 : Hang min Jo, Eun Young Kim, Wang Young Rhee, Jae-Hwang Jeong, Sang Yoon Nam, Beom Jun Lee
ㆍ저자명 : Hang min Jo, Eun Young Kim, Wang Young Rhee, Jae-Hwang Jeong, Sang Yoon Nam, Beom Jun Lee
목차
IntroductionMaterials and Methods
Materials
Animals and experimental diets
Experimental design
Assay of aberrant crypt foci (ACF)
Thiobarbituric acid reactive substances (TBARS) assay
Histopathological examination
Statistical analysis
Results
Changes in body weight
TBARS value in feces
Histopathology
Discussion
References
영어 초록
Globally, colon cancer is increased gradually and known as one of the major causes of cancer death. Stevia, a substitute of sugar, is known to have many components including alpha-tocopherol and anthocyanin etc, as antioxidants. This study's purpose is to investigate whether stevia plant extract can have a protective effect against colon carcinogenesis induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in mice. Total 30 male ICR mice were divided into 2 groups; AOM/DSS treatment (control group), AOM/DSS + stevia extract (0.5%, in drinking water). After acclimation for 1 week, five weeks old mice received three intraperitoneal AOM (10 mg/kg b.w.) injections weekly for 3 weeks (0–2nd weeks of the experiment) and 2% DSS as drinking water for the next one week. AIN-76A purified rodent diet and 0.5% stevia extract water were supplied to the animals for 6 weeks. The colons of mice were collected and the number of aberrant crypt foci (ACF) and aberrant crypts (ACs) in colonic mucosa were counted after staining with methylene blue. Malondialdehyde (MDA) concentration in feces were determined. The numbers of ACF and ACs were significantly (p<0.01) decreased in stevia-treated group compared with the control group. The MDA concentration in feces was also significantly (p<0.01) decreased in stevia-treated group compared with the control group. In histopathology of colonic epithelium, hyperplasia of colonic epithelium was less observed in steviatreated group. These results indicate that stevia has a protective effect against colon carcinogenesis induced by AOM/DSS in mice and further study needs to illustrate the protective mechanisms.참고 자료
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