면류에서 HPLC를 이용한 데옥시니발레놀 분석법의 검증과 불확도 산정
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국식품위생안전성학회
ㆍ수록지정보 : 한국식품위생안전성학회지 / 26권 / 2호
ㆍ저자명 : 옥현이, 장현주, 강영운, 김미혜, 전향숙
ㆍ저자명 : 옥현이, 장현주, 강영운, 김미혜, 전향숙
영어 초록
An isocratic high performance liquid chromatography (HPLC) method for routine analysis of deoxynivalenol in noodles was validated and estimated the measurement uncertainty. Noodles (dried noodle and ramyeon) were analyzed by HPLC-ultraviolet detection using immunoaffinity column for clean-up. The limits of detection (LOD) and quantification (LOQ) were 7.5 μg/kg and 18.8 μg/kg, respectively. The calibration curve showed a good linearity, with correlation coefficients r² of 0.9999 in the concentration range from 20 to 500 μg/kg. Recoveries and Repeatabilities expressed as coefficients of variation (CV) spiked with 200 and 500 μg/kg were 82 ± 2.7% and 87 ± 1.3% in dried noodle, and 97 ± 1.6% and 91 ± 12.0% in ramyeon, respectively. The uncertainty sources in measurement process were identified as sample weight, final volume, and sample concentration in extraction volume as well as components such as standard stock solution, working standard solution, 5 standard solutions, calibration curve,matrix, and instrument. Deoxynivalenol concentration and expanded uncertainty in two matrixes spiked with 200 μg/kg and 500 μg/kg were estimated to be 163.8 ± 52.1 and 435.2 ± 91.6 μg/kg for dried noodle, and 194.3 ± 33.0 and 453.2 ± 91.1 μg/kg for ramyeon using a coverage factor of two which gives a level of statistical confidence with approximately 95%. The most influential component among uncertainty sources was the recovery of matrix, followed by calibration curve.참고 자료
없음"한국식품위생안전성학회지"의 다른 논문
학교 주변 어린이기호식품의 미생물학적 오염도 평가4페이지
유해물질 관리정책의 과학화11페이지
내독소인 LPS로 처치된 흰쥐에 대한 막걸리의 항산화 활성효과5페이지
서울시 일부 제과점 제품의 지방산 조성 및 트랜스지방 함량 분석6페이지
손의 미생물 오염도의 경시적 변화 - 조리종사자를 중심으로6페이지
Betulinic Acid, a Naturally Occurring Triterpene found ..4페이지
생체시료 중 멜라민 화합물의 미량분석법 개발연구17페이지
온도와 시간을 주요 변수로한 훈제연어에서의 Listeria monocytogenes 성장예측모델5페이지
Detection of Escherichia coli O157:H7, Listeria monocyt..6페이지
차전초 추출물을 투여한 랫드에서의 Fe-NTA 유발 산화스트레스에 대한 신장보호 효과7페이지