Microsatellite Markers를 이용한 칡소의 유전적 다형성 분석

서지정보

ㆍ발행기관 : 강원대학교 동물자원공동연구소 ㆍ수록지정보 : 동물자원연구 / 21권 / 2호
ㆍ저자명 : 박새롬, 이선영, 박연수, 송영한, 이성진

목차

ABSTRACT I. 서론 II. 재료 및 방법 1. 공시재료 2. Microsatellite marker의 PCR증폭 3. Microsatellite 유전자형 분석 4. 통계분석 III. 결과 및 고찰 IV. 요약 사사 V. 인용문헌

영어 초록

There are four (yellow, brindle, black, black in Jeju) kinds of native cattle in Korea. But only twelve hundred Korean brindle cattle (KBC) are remaining in limited areas of Korea and the genetic lineage, diversity, polymorphisms of KBC has not been identified. To analysis genetic polymorphism of KBC, 33 KBC were characterized using 11 microsatellite markers. Size of microsatellite marker was decided using Gene Mapper software after analysis ABI 3130XL. The average of allele numbers of KBC was 6.7 in this study, but that of Hanwoo was 10.0 in our previous report. The observed and expected heterozygosities of KBC were 0.719 and 0.738 but those of Hanwoo were 0.751 and 0.760 in our previous report. Also polymorphism information content (PIC) values were average 0.690 in KBC but 0.725 in Hanwoo. These results demonstrate that genetic polymorphism of KBC have decreased because the population was limited.

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