Peripheral Cellular Mechanisms of Artemin-induced Thermal Hyperalgesia in Rats
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 42권 / 1호
ㆍ저자명 : Hye-Jin Kim, Kui-Ye Yang, Min-Kyung Lee, Min-Kyoung Park, Jo-Young Son, Jin-Sook Ju, Dong-Kuk Ahn
목차
서 론
재료 및 방법
실험동물과 수술
안면통증의 평가
실험 프로토콜
실험에 사용한 약물
통계분석
결 과
고 찰
Acknowledgements
Conflict of interest
References
영어 초록
In the present study, we investigated the role of peripheral ionotropic receptors in artemin-induced thermal hyperalgesia in the orofacial area. Male Sprague-Dawley rats weighting 230 to 280 g were used in the study. Under anesthesia, a polyethylene tube was implanted in the subcutaneous area of the vibrissa pad, which enabled drug-injection. After subcutaneous injection of artemin, changes in air-puff thresholds and head withdrawal latency time were evaluated. Subcutaneous injection of artemin (0.5 or 1 μg) produced significant thermal hyperalgesia in a dose-dependent manner. However, subcutaneous injection of artemin showed no effect on air-puff thresholds. IRTX (4 μg), a TRPV1 receptor antagonist, D-AP5 (40 or 80 μg), an NMDA receptor antagonist, or NBQX (20 or 40 μg), an AMPA receptor antagonist, was injected subcutaneously 10 min prior to the artemin injection. Pretreatment with IRTX and D-AP5 significantly inhibited the artemin-induced thermal hyperalgesia. In contrast, pretreatment with both doses of NBQX showed no effect on artemin-induced thermal hyperalgesia. Moreover, pretreatment with H-89, a PKA inhibitor, and chelerythrine, a PKC inhibitor, decreased the artemin-induced thermal hyperalgesia. These results suggested that artemin-induced thermal hyperalgesia is mediated by the sensitized peripheral TRPV1 and NMDA receptor via activation of protein kinases.
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