Xylitol Mitigate Neutrophil Inflammatory Response Against Porphyromonas gingivalis Infection
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 43권 / 3호
ㆍ저자명 : Hee Sam Na, YuRi Song, Yoon Hee Choi, Jin Chung
ㆍ저자명 : Hee Sam Na, YuRi Song, Yoon Hee Choi, Jin Chung
목차
IntroductionMaterials and Methods
Bacterial culture
Animal study
Flow cytometry analysis
Neutrophil isolation and Cell treatment
Cytokine analysis
Statistics
Results
P. gingivalis induces recruitment of neutrophils andinduces inflammatory cytokine production.
P. gingivalis-induces cytokine production in neutrophilsisolated from bone marrow and peritoneal cavity.
Xylitol mitigates P. gingivalis-induced cytokineproduction in neutrophils isolated from bone marrow.
Discussion
References
영어 초록
breakdown of tooth-supporting tissues, producing dentition loss. Porphyromonas gingivalis (P. gingivalis), a Gramnegative anaerobic rod, is one of the major pathogens associated with periodontitis. Neutrophils are first line defense cells in the oral cavity that play a significant role in inflammatory response. Xylitol is a known anti-caries agent and has anti-inflammatory effects. In this study, we conducted experiments to evaluate anti-inflammatory effects of xylitol on P. gingivalis infected neutrophils for possible usage in prevention and treatment of periodontal infections. P. gingivalis was intraperitoneally injected and peritoneal lavage was collected for cytokine determination. For in vitro study, neutrophils were collected from mouse peritoneal cells after zymosan injection or bone marrow cells. Neutrophils were stimulated with live P. gingivalis and ELISA was used to determine the effect of xylitol on P. gingivalis induced cytokine production. IL-1β, IL-6, TNF-α concentration and neutrophil population in the peritoneal lavage was increased in P. gingivalis-infected mouse. Peritoneal cells infected with live P. gingivalis revealed significantly increased production of IL-1β, IL-6 and TNF-α at multiplicity of infection of 10. Neutrophils from bone marrow and peritoneal lavage revealed increased production of IL-1β, IL-6 and TNF-α. Xylitol significantly mitigated P. gingivalis induced cytokine production in neutrophils. Findings indicate that xylitol is an anti-inflammatory agent in neutrophils infected with live P. gingivalis, that suggests its use in periodontitis management.참고 자료
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