Induction of anti-aquaporin 5 autoantibodies by molecular mimicry in mice
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 45권 / 4호
ㆍ저자명 : Ahreum Lee, Youngnim Choi
ㆍ저자명 : Ahreum Lee, Youngnim Choi
목차
IntroductionMaterials and Methods
1. 세균 배양
2. 펩타이드
3. 생쥐 면역화
4. 항체 정량96-
5. 통계 분석
Results
Discussion
Acknowledgements
Conflicts of Interest
References
영어 초록
Molecular mimicry is the most common mechanism that breaches self-tolerance. We previously identified autoantibodies to aquaporin-5 (AQP5) in the sera of patients with Sjögren’s syndrome and found that the aquaporin of Prevotella melaninogenica (PmAqp), an oral commensal, is highly homologous to human AQP5. This study aimed to test whether PmAqp can induce anti-AQP5 autoantibodies via molecular mimicry. From the amino acid sequence of PmAqp, an immunizing peptide; i.e., PmE-L, was designed, which contained both the B cell epitope “E” and T cell epitope. C57BL/6 and BALB/c mice were subcutaneously immunized with linear or cyclic forms of PmE-L emulsified in incomplete Freund’s adjuvant. The concentrations of the antibodies in sera were measured using enzymelinked immunosorbent assays. Both linear and cyclic PmE-L induced high levels of antibodies against not only the immunized peptides but also autoantibodies against AQP5E and antibodies against PmE, a Pm homolog of AQP5E. In C57BL/6 mice; however, the cyclic form of PmE-L was more efficient than the linear form in inducing autoantibodies against AQP5E that contained a cyclic epitope. The levels of anti-PmE antibodies and anti-AQP5E autoantibodies showed a strong positive correlation (r = 0.95, p < 0.0005), suggesting molecular mimicry. Collectively, the mice produced anti-AQP5E autoantibodies in response to a PmAqp-derived peptide. This model proved to be useful for studying the mechanisms of autoantibody production by molecular mimicry.참고 자료
없음"International Journal of Oral Biology"의 다른 논문
Role of microglial activation on neuronal excitability ..7페이지
Antitumor effects of octyl gallate on hypopharyngeal ca..7페이지
High-salt and glucose diet attenuates alveolar bone rec..7페이지
Increased SOX2 expression in three-dimensional sphere c..7페이지
Alteration of cellular events in tooth development by c..7페이지
Green tea polyphenol (–)-epigallocatechin-3-gallate pre..11페이지
L-ascorbic acid induces apoptosis in human laryngeal ep..10페이지
High concentration of calcium represses osteoblast diff..7페이지
Anticancer effects of D-pinitol in human oral squamous ..10페이지
Inhibitory effects of Coptis chinensis extract on the g..9페이지