Homer2 regulates amylase secretion via physiological calcium oscillations in mouse parotid gland acinar cells
(주)코리아스칼라
- 최초 등록일
- 2023.04.03
- 최종 저작일
- 2020.06
- 6페이지/
어도비 PDF
- 가격 4,000원
![할인쿠폰받기](/images/v4/document/ico_det_coupon.gif)
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 45권 / 2호
ㆍ저자명 : Namju Kang, Jung Yun Kang, Dong Min Shin, Yu-Mi Yang
목차
Introduction
Materials and Methods
1. 재료
2. 실험동물
3. 이하선의 포상세포 분리
4. 세포 내 칼슘 농도([Ca2+]i) 측정
5. 아밀라아제 분비량 측정
6. 실험자료의 통계 분석
Results
1. 작용제에 의한 세포 내 칼슘의 변화 양상
2. 칼슘 의존적 아밀라아제의 분비기능 변화
Discussion
References
영어 초록
The salivary glands secrete saliva, which plays a role in the maintenance of a healthy oral environment. Under physiological conditions, saliva secretion within the acinar cells of the gland is regulated by stimulation of specific calcium (Ca2+) signaling mechanisms such as increases in the intracellular Ca2+ concentration ([Ca2+]i) via storeoperated Ca2+ entry, which involves components such as Orai1, transient receptor potential (TRP) canonical 1, stromal interaction molecules, and inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs). Homer proteins are scaffold proteins that bind to G protein-coupled receptors, IP3Rs, ryanodine receptors, and TRP channels. However, their exact role in Ca2+ signaling in the salivary glands remains unknown. In the present study, we investigated the role of Homer2 in Ca2+ signaling and saliva secretion in parotid gland acinar cells under physiological conditions. Deletion of Homer2 (Homer2−/−) markedly decreased the amplitude of [Ca2+]i oscillations via the stimulation of carbachol, which is physiologically concentrated in parotid acinar cells, whereas the frequency of [Ca2+]i oscillations showed no difference between wild-type and Homer2−/− mice. Homer2−/− mice also showed a significant decrease in amylase release by carbachol in the parotid gland in a dose-dependent manner. These results suggest that Homer2 plays a critical role in maintaining [Ca2+]i concentration and secretion of saliva in mouse parotid gland acinar cells.
참고 자료
없음
"International Journal of Oral Biology"의 다른 논문