Regulation of the expression and function of TRPCs and Orai1 by Homer2 in mouse pancreatic acinar cells
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- 2023.04.03
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- 2021.09
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 46권 / 3호
ㆍ저자명 : Jung Yun Kang, Namju Kang, Yu-Mi Yang
목차
Introduction
Materials and Methods
1. 재료
2. 실험동물
3. 췌장의 선세포 분리
4. 중합효소 연쇄 반응(polymerase chain reaction, PCR)분석
5. [Ca2+]i 측정
6. 실험자료의 통계 분석
Results
1. 칼슘 유입을 매개하는 채널들의 발현 양상
2. 특정 억제제에 의한 수용체-의존성 칼슘 유입의 조절 변화
Discussion
References
영어 초록
Under physiological conditions, calcium (Ca2+) regulates essential functions of polarized secretory cells by the stimulation of specific Ca2+ signaling mechanisms, such as increases in intracellular Ca2+ concentration ([Ca2+]i) via the store-operated Ca2+ entry (SOCE) and the receptor-operated Ca2+ entry (ROCE). Homer proteins are scaffold proteins that interact with G protein-coupled receptors, inositol 1,4,5-triphosphate (IP3) receptors, Orai1-stromal interaction molecule 1, and transient receptor potential canonical (TRPC) channels. However, their role in the Ca2+ signaling in exocrine cells remains unknown. In this study, we investigated the role of Homer2 in the Ca2+ signaling and regulatory channels to mediate SOCE and ROCE in pancreatic acinar cells. Deletion of Homer2 (Homer2–/–) markedly increased the expression of TRPC3, TRPC6, and Orai1 in pancreatic acinar cells, whereas these expressions showed no difference in whole brains of wild-type and Homer2–/– mice. Furthermore, the response of Ca2+ entry by carbachol also showed significant changes to the patterns regulated by specific blockers of SOCE and ROCE in pancreatic acinar cells of Homer2–/– mice. Thus, these results suggest that Homer2 plays a critical role in the regulatory action of the [Ca2+]i via SOCE and ROCE in mouse pancreatic acinar cells.
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