Optimization of residual analysis of streptomycin and dihydrostreptomycin in swine plasma, urine and tissues using LC-MS/MS
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서지정보
ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
ㆍ수록지정보 : 예방수의학회지 / 46권 / 3호
ㆍ저자명 : Sunjin Park, Chung-Oui Hong, Hyobi Kim, Yu-Jung Kim, Seon-Young Lee, Inhae Jeon, Kwang-jick Lee, Moon Her, Sung-Won Park
ㆍ저자명 : Sunjin Park, Chung-Oui Hong, Hyobi Kim, Yu-Jung Kim, Seon-Young Lee, Inhae Jeon, Kwang-jick Lee, Moon Her, Sung-Won Park
목차
Abstract서 론
재료 및 방법
시약 및 재료
표준원액 및 표준용액의 조제
시료 전처리
기기분석 조건
결 과
고 찰
REFERENCES
영어 초록
This study aims to establish a modified analytical method with sensitivity and reliability for streptomycin (STP) and dihydrostreptomycin (DHS) of residues level in pig tissues, plasma and urine by LC-MS/MS on the basis of previous studies. The mass parameters of quantitative and qualitative ions for STP and DHS were optimized using multiple reaction monitoring in positive mode. The separation of compounds was conducted using BEH Amide column according to material’s characteristics. The analytes in plasma were extracted with only organic solvents. In muscle and kidney, KH2PO4 buffer solution containing 2% CCl3COOH and EDTA-Na was used as extraction solvent. The WCX cartridege was selected as SPE cartridge in considering high recoveries for STP and DHS. The analytes in urine were extracted by organic solvents with acid and addition of EDTA. The limits of detection (LODs) in STP and DHS ranged 0.45~3.66 μg/kg and 0.22~0.78 μg/kg, respectively. The limits of quantification (LOQs) were 1.35~11.10 μg/kg in STP and 0.66~2.36 μg/kg in DHS. The recoveries (%) were 94.29~104.5% in STP and 92.32~108.45% in DHS except for plasma with lower values (61.45/68.5%, respectively). In the precision evaluation, the coefficient of variation (CV, %) of STP showed <10.50% on intra-day and <18.04% on inter-day. The CV (%) of DHS showed <8.42% on intra-day, whereas <17.98% on inter-day. The modified method is reliable for continuous residual monitoring in pig to ensure food safety for consumer’s health. In addition, this method could be used in study relation to residue depletion and pharmacokinetics of veterinary drug.참고 자료
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