Effect of Insulin Supplement on Development of Porcine Parthenogenetic Embryos
(주)코리아스칼라
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- 2016.09.03
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서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 31권 / 2호
ㆍ저자명 : Youngkwang Yu, Pantu Kumar Roy, Kyuhong Min, Seungwon Na, Euncheol Lee, Ghangyong Kim, Xun Fang, MB Salih, Jongki Cho
목차
INTRODUCTION
MATERIALS and METHODS
1. In vitro maturation of porcine oocytes
2. Production of parthenogenetic embryos
3. Experimental plans
4. Statistical analysis
RESULTS
1. Effects of insulin on embryonic development in vitro maturationof porcine oocytes
2. Developments of porcine parthenogenetic embryos
3. Blastocysts cell number of porcine parthenogenetic pig oocytes
DISCUSSION
영어 초록
This study is performed to evaluate the effect of insulin in the porcine parthenogenetic embryo development. In porcine embryo culture, insulin is helpful factor in the process of embryo development. To identify this, insulin is used in pig embryos development. Therefore, this study was performed to investigate the effect of insulin on early embryonic development in pigs. For that, insulin positive or negative (0, 10 ug/mL) was supplemented in the porcine IVM media and then compared two groups divided by the cytoplasm of the black groups and white ring groups based on the distribution of lipid material of the cell cytoplasm in microscope. In maturation rates of porcine oocytes, significant higher black group rates were shown in the insulin positive groups compared with other groups (56.0±2.1 vs 46.2±0.3). In the embryo culture, black groups were showed the significant higher cleavage rates (82.1±0.8, 78.3±0.1 vs 63.2±0.3, 63.4±0.0), and blastocyst formation rates (15.5±3.6, 16.6±0.4 vs 11.7±1.3, 7.4±0.2) regardless of whether the addition of insulin. Also, black groups were showed higher cell number of blastocyst (33.2±2.5, 35.5±2.6 vs 31.2±2.1, 31.3±2.2). In conclusion, supplement of insulin producing black group in vitro maturation, it was effective in vitro maturation and embryonic development of pig embryos.
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