Analysis of porcine macrophage immune response to antigenic molecules and short chain fatty acids
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서지정보
ㆍ발행기관 : 충북대학교 동물의학연구소
ㆍ수록지정보 : Journal of Biomedical Research / 16권 / 2호
ㆍ저자명 : Na-Eun Han, Eun-Joo Lee, Kwan-Sik Park, In-Sook Jeon, Hak-Kyo Lee, Ki-Duk Song, Joong-Kook Choi
ㆍ저자명 : Na-Eun Han, Eun-Joo Lee, Kwan-Sik Park, In-Sook Jeon, Hak-Kyo Lee, Ki-Duk Song, Joong-Kook Choi
목차
IntroductionMaterials and Methods
Cell Culture and Reagents
DNA Microarray
Reversre Transcriptase (RT) PCR
Results
Analysis of Differentially Expressed Porcine Genes
Gene Expression Analysis of Porcine Genes identified in DNA microarray by RT-PCR
Expression Profile of Porcine Antimicrobial Genes
Discussion
Acknowledgements
ORCID
References
영어 초록
Macrophages play an important role in both the innate and adaptive immune responses. These include phagocytosis, killing of microorganisms, antigen presentation, and induction of immune cytokines and antimicrobial genes. Macrophage activity is reported to be controlled by diverse exogenous antigenic or endogenous metabolic molecules, and the underlying mechanisms are well documented in human and mouse macrophage cells. Bacterial lipopolysaccharide (LPS) is known to be one of the most potent stimuli activating macrophages through the toll like receptor 4 (TLR4) signaling pathway. There are other antigenic molecules, such as muramyl dipeptide (MDP) and outer membrane protein A (OmpA), that are also known to activate immune cells. On the other hand, short chain fatty acids (SCFAs) such as acetate and butyrate are produced by gut microbiota and control host energy metabolism and signal transduction through GPR receptors. However, there are few studies demonstrating the effects of these molecules in macrophages from domestic animals, including domestic pigs. In this study, we attempted to characterize gene expression regulation in porcine macrophages (PoM2, Pig Monocytes clone 2) following treatment with LPS, MDP, OmpA, and two short chain fatty acids using porcine genome microarray and RT-PCR techniques. A number of novel porcine genes, including anti-microbial peptides and others, appeared to be regulated at the transcriptional level. Our study reports novel biomarkers such as SLC37A2, TMEN184C, and LEAP2 that are involved in the porcine immune response to bacterial antigen LPS and two short chain fatty acids.참고 자료
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