Caffeic Acid Phenethyl Ester-Mediated Cytotoxicity in MC-3 Human Mucoepidermoid Carcinoma Cell Line
(주)코리아스칼라
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- 2016.04.02
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서지정보
ㆍ발행기관 : 대한구강악안면병리학회
ㆍ수록지정보 : 대한구강악안면병리학회지 / 38권 / 6호
ㆍ저자명 : 유현주, 조남표, 조성대
목차
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Cell culture and chemical treatment
2. Antibodies
3. Cell viability assay
4. DAPI staining
5. Live/Dead assay
6. Western blot analysis
7. Statistical analysis
Ⅲ. RESULTS
1. CAPE reduces cell viability and causesapoptosis and cytotoxicity in MC-3 cells
2. Extrinsic apoptotic pathway is involved inCAPE-mediated apoptosis and it also activatesAKT and ERK signaling in MC-3 cells
IV. DISCUSSION
V. REFERENCES
영어 초록
Caffeic acid phenethyl ester (CAPE), a component of propolis, was reported to possess anti-inflammatory, anti-bacterial, anti-viral, and anti-tumor activities. Our aim was to investigate the effect of CAPE on apoptosis in cultured human mucoepidermoid carcinoma (MEC) cell line, MC-3. Apoptotic effects of CAPE were measured by cell viability assays, Western blotting, 4’-6-diamidino-2-phenylindole (DAPI) staining and Live/Dead assay. The result of cell viability assay showed that CAPE displayed a strong growth-inhibitory effect in a concentration-dependent manner against MC-3 cells. Consumption of CAPE resulted in pronounced increase in the cleavage of caspase-3 and PARP, induced nuclear condensation and fragmentation and clearly increased the number of dead cells in MC-3 cells. CAPE also caused the increase in truncated Bid (t-Bid) and the cleavage of caspase-8 and this phenomenon was regulated by death receptor 5 (DR5). In addition, Phosphorylation of AKT and ERK were downregulated by CAPE. Taken together, these results suggest that CAPE is a potent apoptosis-inducing agent in MC-3 cells.
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