Effects of Insulin, Transferrin and Selenium(ITS) on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 4호
ㆍ저자명 : Yan Shi Quan, Kenji Naruse, Baek Chul Kim, Hong Rye Kim, Rong Xun Han, Su Min Choi, Chang Sik Park, Dong Il Jin

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Preparation of Oocytes Production of Porcine Parthenogenetic Embryos Preparation of Fetal Fibroblasts Production of Porcine Nuclear Transfer embryos Evaluation of Developmental Ability of Parthenogenetic and Reconstructed Embryos Experimental Design Statistical Analysis RESULTS DISCUSSION REFERENCES

영어 초록

Insulin, transferrin and selenium (ITS) complex is reported to improve in vitro development of oocytes and embryos. This study was carried out to investigate the effects of ITS during in vitro culture (IVC) of porcine parthenogenetic and nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. The electrically activated oocytes were cultured in Porcine Zygote Medium (PZM-3) with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 7 days. Also, the electrically activated reconstructed embryos were cultured in PZM-3 with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 6 days. Addition of ITS to culture medium did not affect development of porcine parthenogenetic embryos in vitro. To test the effect of ITS on the in vitro development of porcine NT embryos, factorial experiments were also performed for in vitro maturation (IVM) medium (TCM-199) with or without 1% ITS and culture medium (PZM-3) with or without 0.5% ITS. Addition of 0.5% ITS to culture medium increased (p<0.05) the proportion of NT blastocysts compared with non-treated group. In contrast, addition of 1% ITS to culture medium was ineffective or had a detrimental effect. Also, addition of ITS only to maturation medium increased (p<0.05) the percentage of NT blastocysts formation compared with the control group. In conclusion, addition of ITS to IVM or IVC medium could improve subsequent blastocyst development of porcine NT embryos.

참고 자료

없음