In Vitro Expansion of Homogeneous Neural Precursor Cells Derived from Human Embryonic Stem Cells

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 4호
ㆍ저자명 : Deuk-Chae Na, Sehee Kim, Won-Ik Choi, Hyun-Jin Hwang, Inho Han, Jae-hwan Kim, Keun-Hong Park, Hyung-Min Chung, Seong-Jun Choi

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Culture of Human ES Cells Neural Differentiation of Human ES Cells RT-PCR Analysis Cell Proliferation Analysis Immunochemical Analysis RESULTS Expansion and Characterization of Neural Precursor Cells DISCUSSION REFFRENCES

영어 초록

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the capacity to differentiate into various types of cells in the body. Hence, these cells may potentially be an indefinite source of cells for cell therapy in various degenerative diseases including neuronal disorders. For clinical applications of human ES cells, directed differentiation of these cells would be necessary. The objective of this study is to develop the culture condition for the expansion of neural precursor cells derived from human ES cells. Human ES cells were able to differentiate into neural precursor cells upon a stepwise culture condition. Neural precursor cells were propagated up to 5000-fold in cell numbers over 12-week period of culture and evaluated for their characteristics. Expressions of sox1 and pax6 transcripts were dramatically up-regulated along the differentiation stages by RT-PCR analysis. In contrast, expressions of oct4 and nanog transcripts were completely disappeared in neural precursor cells. Expressions of nestin, pax6 and sox1 were also confirmed in neural precursor cells by immunocytochemical analysis. Upon differentiation, the expanded neural precursor cells differentiated into neurons, astrocytes, and oligodendrocytes. In immunocytochemical analysis, expressions of type III β-tubulin and MAP2ab were observed. Presence of astrocytes and oligodendrocytes were also confirmed by expressions of GFAP and O4, respectively. Results of this study demonstrate the feasibility of long-term expansion of human ES cell-derived neural precursor cells in vitro, which can be a potential source of the cells for the treatment of neurodegenerative disorders.

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