In Vitro Development and Chromosome Constitution of Porcine Parthenotes following Different Activation Treatments
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 4호
ㆍ저자명 : Hae-Joo Wi, Dae-Jin Kwon, Joo-Hee Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
ㆍ저자명 : Hae-Joo Wi, Dae-Jin Kwon, Joo-Hee Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS In Vitro Maturation of Oocytes Parthenogenetic Activation In Vitro Culture Cytological Analysis Statistical Analysis RESULTS Ddevelopment of Parthenotes Ploidy of One-Cell Parthenotes Ploidy of Parthenogenetic Blastocysts DISCUSSION REFFRENCES영어 초록
This study was conducted to examine the protein kinase inhibitors, 6-dimethylaminopurine (DMAP) and cycloheximide (CHXM) on the development and chromosome constitution of porcine parthenogenetic embryos. In vitro matured oocytes were activated by electric stimuli (ES) or a combination of ES with culture in 2 mM DMAP or 10 μg/ml CHXM for 4 hr. Activated oocytes were cultured in PZM-3 for 6 days. Some 1-cell embryos and blastocysts were fixed by air dry method to analyze the chromosome constitutions and/or total cell number. Blastocyst development of DMAP-treated group (26.7%) was significantly higher (p<0.05) than those of CHXM-treated and ES control groups. Ploidy in 1-cell stage embryos was not different among groups (77.3 to 81.0%), however, proportion of diploid chromosome constitutions was high in DMAP-treated group (61.9%, p<0.05). In the blastocyst stage, proportion of diploid chromosome plates was significantly high in DMAP-treated group (64.2%, p<0.05), and proportion of abnormal chromosome plates was higher in CHXM-treated group (36.6%, p<0.05) than DMAP-treated group (28.3%,). Proportion of embryos with abnormal chromosome constitutions was slightly increased by DMAP (40.0%) and CHXM (42.1%) treatment due to the increasing of mixoploid (47.4 and 52.0%). The present study shows that the DMAP treatment increase the development of porcine parthenotes. However, parthenogenetic activation by ES or combined treatment with ES and DMAP or CHXM detrimentally affects the chromosome constitutions of porcine parthenotes during early embryonic development, leads to increased abnormal ploidy in the blastocyst stage.참고 자료
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