Effects of Oocyte Maturation Age and Activation Condition on the Development of Porcine Parthenogenetic Embryos
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 2호
ㆍ저자명 : Dae-Jin Kwon, Joo-Hee Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
ㆍ저자명 : Dae-Jin Kwon, Joo-Hee Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
목차
ABSTRACT INTRIDUSTION MATERIALS AND METHODS In Vitro Maturation of Oocytes Evaluation of Nuclear Maturation MPF Kinase Assay Parthenogenetic Activation In Vitro Culture Statistical Analysis RESULTS Maturation Pattenrs of Porcine Oocytes cdc2 Kinase Activity Development of Parthenotes DISCUSSION REFERENCES영어 초록
This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for 30 sec; ES), 2) double electric stimulations (1.5 kV/cm for 30 sec, followed by 1.0 kV/cm for 50 sec after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6~7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.참고 자료
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