Periodontopathogen LPSs Regulate MicroRNA Expression in Human Gingival Epithelial Cells
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 36권 / 3호
ㆍ저자명 : Jin Chung, Hwa Sun Lee, Hee Sam Na, So-Yeon Jeong, Sung-Hee Jeong, Hae-Ryoun Park
ㆍ저자명 : Jin Chung, Hwa Sun Lee, Hee Sam Na, So-Yeon Jeong, Sung-Hee Jeong, Hae-Ryoun Park
영어 초록
Periodontitis results from the activation of host immune and inflammatory defense responses to subgingival plaque bacteria, most of which are gram-negative rods with lipopolysaccharides (LPSs) in their cell walls. LPSs have been known to induce proinflammatory responses and recently it was reported also that they induce the expression of microRNAs(miRNAs) in host cells. In our current study therefore, we aimed to examine and compare the miRNA expression patterns induced by the LPSs of major periodontopathogens in the human gingival epithelial cell line, Ca9-22. The cells were treated with 1 μg/ml of E. coli (Ec) LPS or 5 μg/ml of an LPS preparations from four periodontopathogens Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) for 24 h. After small RNA extraction from the treated cells, miRNA microarray analysis was carried out and characteristic expression profiles were observed. Fn LPS most actively induced miRNAs related to inflammation, followed by Aa LPS, Pi LPS, and Ec LPS. In contrast, Pg LPS only weakly activated miRNAs related to inflammation. Among the miRNAs induced by each LPS, miR-875-3p, miR-449b, and miR-520d-3p were found to be commonly up-regulated by all five LPS preparations, although at different levels. When we further compared the miRNA expression patterns induced by each LPS, Ec LPS and Pi LPS were the most similar although Fn LPS and Aa LPS also induced a similar miRNA expression pattern. In contrast, the miRNA profile induced by Pg LPS was quite distinctive compared with the other bacteria. In conclusion, miR-875- 3p, miR-449b, and miR-520d-3p miRNAs are potential targets for the diagnosis and treatment of periodontal inflammation induced by subgingival plaque biofilms. Furthermore, the observations in our current study provide new insights into the inflammatory miRNA response to periodontitis.참고 자료
없음"International Journal of Oral Biology"의 다른 논문
Apoptotic Effects of A Cisplatin and Eugenol Co-treatme..8페이지
Protective Effects of Alpinia katsumadai Extract Agains..6페이지
Participation of Peripheral P2X Receptors in Orofacial ..6페이지
The Epithelial-Mesenchymal Transition During Tooth Root..7페이지
S Phase Cell Cycle Arrest and Apoptosis is Induced by E..6페이지