Hyperglycemia Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 37권 / 2호
ㆍ저자명 : Yong-Nan Xu, Ying-Hua Li, Sung Hyun Lee, Jung-Woo Kwon, Seul Ki Lee, Young-Tae Heo, Xiang-Shun Cui, Nam-Hyung Kim
ㆍ저자명 : Yong-Nan Xu, Ying-Hua Li, Sung Hyun Lee, Jung-Woo Kwon, Seul Ki Lee, Young-Tae Heo, Xiang-Shun Cui, Nam-Hyung Kim
목차
ABSTRACTINTRODUCTION
MATERIALS AND METHODS
ocyte Collection and In Vitro Maturation Embryo Culture
Terminal Deoxynucleotidyl Transferase-Mediated dUTPNick-End Labeling (TUNEL)
AssaTerminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL)
Assayy
Mitochondrial Staining and Image Analysis
Immunofluorescence Staining
Real Time Reverse Transcription Polymerase Chain Reaction(Real Time RT-PCR)
Statistical Analysis
RESULTS
Effect of Different Concentrations of Glucose on PorcineEmbryos Developing In Vitro
Glucose Concentration Affects Cell Number and Apoptosisin The Blastocyst Stage
Hyperglycemia Affects Mitochondrial Distribution but not Nuclear DNA-Encoded Mitochondrial Gene Expressionin Blastocysts
Effect of Hyperglycemia on Autophagy and Autophagy-Related Gene Expression in Blastocysts
DISCUSSION
REFERENCES