배양액 및 산소농도가 돼지 체외수정란의 발달에 미치는 영향

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 22권 / 3호
ㆍ저자명 : 최창용, 조상래, 최선호, 김현종, 한만희, 강다원, 신용원, 한재희, 손동수

한국어 초록

본 연구는 돼지 체외수정란 생산효율을 향상시켜 돼지의 품종개량, 형질전환 돼지생산 등과 멸실위험에 처해 있는 유전자원의 보존을 위한 기술로 활용하기 위해 미성숙 난포란의 적정 체외성숙 시간을 알아보고, 배양액의 종류 및 체외 배양시의 산소 농도에 따른 체외수정란의 생산 효율을 확인한 결과는 다음과 같다. 1. 돼지 미성숙 난포란의 체외성숙 시간별 제2감수분열중기(M II)까지 성숙된 비율이 체외성숙 38, 40, 42시간째에 각각 61.1%, 42.9%

영어 초록

During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen (), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% , in NCSU-23), the mean cell number per blastocyst was . These results showed that medium and concentration had no significant effect on the development of porcine embryos.

참고 자료

없음