Toxicity and Biomedical Imaging of Fluorescence-Conjugated Nanoparticles in Hematopoietic Progenitor Cells
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Gyesik Min, Dong-Ku Kim
ㆍ저자명 : Gyesik Min, Dong-Ku Kim
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Preparation of Nanoparticles Preparation of Hematopoietic Cells Flow Cytometry Analysis Cell Viability and Proliferation Assay Cell Cycle Analysis Colony-Forming-Cell Ability Assay In Vivo Hematopoietic Reconstitution Activity Statistical Analysis RESULTS Uptake Efficiency into Bone Marrow Hematopoietic Cells Cell Proliferation and Cytotoxic Effects In Vitro Hematopoietic Differential Effects In Vivo Hematopoietic Reconstitution Effects Intracellular Uptake into Hematopoietic Stem/Progenitor Cells DISCUSSION REFERENCES영어 초록
Cellular uptake of nanoparticles for stem cell labeling and tracking is a critical technique for biomedical therapeutic applications. However, current techniques suffer from low intracellular labeling efficiency and cytotoxic effects, which has led to great interest in the development of a new labeling strategy. Using silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (RITC) (SR), we tested the cellular uptake efficiency, biocompatibility, proliferation or differentiation ability with murine bone marrow derived hematopoietic stem/progenitor cells. The bone marrow hematopoietic cells showed efficient uptake with SR with dose or time dependent manner and also provided a higher uptake on hematopoietic stem/progenitor cells. Biocompatibility tests revealed that the SR had no deleterious effects on cell cytotoxicity, proliferation, or multi-differentiation capacities in vitro and in vivo. SR nanoparticles are advantageous over traditional labeling techniques as they possess a high level of cellular internalization without limiting the biofunctionality of the cells. Therefore, SR provides a useful alternative for gene or drug delivery into hematopoietic stem/progenitor cells for basic research and clinical applications.참고 자료
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