돼지 초기수정란에서 Dnmt1o와 Dnmt1s상류 영역의 DNA 메틸화 변화

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 김현미, 김성우, 조상래, 김현, 박재홍, 조재현, 양보석, 고응규

목차

ABSTRACT 서론 재료 및 방법 정자, 난자 및 체외수정란의 회수 체내수정란의 회수 Bisulfite Sequencing에 의한 DNA 메틸화 해석 Pyrosequencing에 의한 DNA 메틸화 해석 결과 초기 배 발달 중 Dnmt1o 상류 영역의 DNA 메틸화 변화 초기 배 발달 중 Dnmt1s 상류 영역의 DNA 메틸화 변화 Bisulfite Sequencing 방법에 의한 체내․체외 배반포에서 DNA 메틸화 분석 고찰 인용문헌

영어 초록

In the present study, we identified differentially methylated region (DMR) upstream of Dnmt1o and Dnmt1s gene in early porcine embryos. Porcine Dnmt1o had at least one DMR which was located between —530 bp to —30 bp upstream from transcription start site of the Dnmt1o gene. DNA methylation analyses of Dnmt1o revealed the DMR to be hypomethylated in oocytes, whereas it was highly methylated in sperm. Moreover, the DMR upstream of Dnmt1o was gradually hypermethylated from oocytes to two cells and dramatically changed in the methylation pattern from four cells to BL stages in an in vivo. In an IVF, the methylation status in the DMR upstream of Dnmt1o was hypermethylated from one cell to eight cells, but demethylated at the Morula and BL stages, indicating that the DNA methylation pattern in the Dnmt1o upstream ultimately changed from stage to stage before the implantation. Next, to elucidate whether DNA methylation status of Dnmt1s upstream is stage-by-stage changed in during porcine early development, we analyzed the dynamics of the DNA methylation status of the Dnmt1s locus in germ cell, or one cell to BL cells. The Dnmt1s upstream was highly methylated in one and eight cells, while less methylated in two, four, morula, and BL cells. Taken together, our data demonstrated that DNA methylation and demethylation events in upstream of Dnmt1o/Dnmt1s during early porcine embryos dramatically occurred, and this change may contribute to the maintenance of genomewide DNA methylation in early embryonic development.

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